Journal: Structure (London, England : 1993)

Article Title: Inhibition of the pneumococcal virulence factor StrH and molecular insights into N-glycan recognition and hydrolysis.

doi: 10.1016/j.str.2011.08.011

Figure Lengend Snippet: Figure 1. The Modular Architecture of StrH from S. pneumoniae (Strain TIGR4) Both catalytic modules are shown in black and the ‘‘unclassified’’ G5 modules in white. The white and gray boxes at the N and C termini, respectively, correspond the secretion signal and the LPXTG cell-wall anchoring motif. Amino acid boundaries of the catalytic domains are indicated above the schematic.

Article Snippet: Gene fragments encoding both GH20A and B modules (GH20A/B, amino acids 181-984) and the individual GH20 catalytic modules (GH20A, residues 181-614; GH20B, residues 627-1039) were amplified by PCR from S. pneumoniae TIGR4 genomic DNA (American Type Culture Collection BAA-334D) using specific primers to introduce a 50 NdeI and 30 XhoI restriction sites (see Table S1 available online for oligonucleotide primer sequences).

Techniques:

Journal: Structure (London, England : 1993)

Article Title: Inhibition of the pneumococcal virulence factor StrH and molecular insights into N-glycan recognition and hydrolysis.

doi: 10.1016/j.str.2011.08.011

Figure Lengend Snippet: Figure 7. Inhibitors of Strh Increase Opsonophagocytic Killing of S. pneumoniae Survival of S. pneumoniae TIGR4 in neutrophil killing assays, showing comparisons of wild-type (filled bars) and Dstrh strain (open bars) in the presence and absence of NGT or PUGNAc. Asterisks above sample bars represent statistical comparison of that sample with the reference, which is the TIGR4 strain with no inhibitor. Statistical differences were analyzed by unpaired Student’s two-tailed t test. Data are mean values compiled from two independent experiments performed in duplicate ± standard error of the mean. *p < 0.05; **p < 0.01; ***p < 0.001. The Dstrh samples with inhibitors were compared with Dstrh in the absence of inhibitors and were found to have p values >0.1 and thus were not significantly different.

Article Snippet: Gene fragments encoding both GH20A and B modules (GH20A/B, amino acids 181-984) and the individual GH20 catalytic modules (GH20A, residues 181-614; GH20B, residues 627-1039) were amplified by PCR from S. pneumoniae TIGR4 genomic DNA (American Type Culture Collection BAA-334D) using specific primers to introduce a 50 NdeI and 30 XhoI restriction sites (see Table S1 available online for oligonucleotide primer sequences).

Techniques: Comparison, Two Tailed Test

Journal:

Article Title: Search for Genes Essential for Pneumococcal Transformation: the RadA DNA Repair Protein Plays a Role in Genomic Recombination of Donor DNA ▿

doi: 10.1128/JB.00573-07

Figure Lengend Snippet: Genes identified by GAF as being involved in pneumococcal transformation

Article Snippet: The resulting DNA fragments were used as a template for an in vitro T7 RNA polymerase reaction (T7 MegaScript; Ambion).

Techniques:

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Antimicrobial Properties of 8-Hydroxyserrulat-14-en-19-oic Acid for Treatment of Implant-Associated Infections

doi: 10.1128/AAC.01735-12

Figure Lengend Snippet: Microorganism strains used in this study

Article Snippet: CFU were determined by plating of aliquots of 10-fold dilutions of bacterial cultures on Mueller-Hinton agar (MHA), followed by 24 h of incubation at 37°C. table ft1 table-wrap mode="anchored" t5 caption a7 Species Strain a Relevant properties Source or reference Staphylococcus aureus Western Samoan Phage Pattern A (WSPPA) * MRSA ST30 13 ME230 * MSSA, isogenic ΔSCC mec mutant of WSPPA M. Ender b SA113 (ATCC 35556) † MSSA, production of PIA-mediated biofilm under aerobic conditions 14 Staphylococcus epidermidis 1457 ‡ Efficient biofilm producer 17 1457 Δ ica ‡ Isogenic Δ ica mutant, devoid of PIA-mediated biofilm 17 Mycobacterium tuberculosis ATCC 27294 (H37Rv) § Rifampin-sensitive strain 15 ATCC 35838 (H37Rv-RIF-R) ‡ Rifampin-resistant strain Streptococcus pyogenes ATCC 19615 § Laboratory strain isolated from human 16 Streptococcus pneumoniae TIGR4 (JNR.7/87) Encapsulated, virulent strain Enterococcus faecalis ATCC 19433 § Laboratory strain Pseudomonas aeruginosa PA01 ¶ Laboratory strain isolated from human Escherichia coli ATCC 25922 Human clinical isolate Candida albicans SC5314 ∥ Laboratory strain isolated from human Candida glabrata t608919 ‡ Clinical isolate This study Candida krusei ATCC 6258 ‡ Reference laboratory strain isolated from human Open in a separate window a Sources: *, B. Berger-Bächi, Zürich, Switzerland; †, F. Götz, Tübingen, Germany; ‡, D. Mack, Swansea, United Kingdom; §, R. Frei, Basel, Switzerland; ¶, U. Jenal, Basel, Switzerland; ∥, S. Leibundgut-Landmann, Zürich, Switzerland. b M. Ender, ETH Zürich, Switzerland (unpublished data).

Techniques: Western Blot, Mutagenesis, Isolation

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Antimicrobial Properties of 8-Hydroxyserrulat-14-en-19-oic Acid for Treatment of Implant-Associated Infections

doi: 10.1128/AAC.01735-12

Figure Lengend Snippet: In vitro susceptibility of Gram-positive and Gram-negative bacteria and fungi to EN4 determined as MICs and MBCs

Article Snippet: CFU were determined by plating of aliquots of 10-fold dilutions of bacterial cultures on Mueller-Hinton agar (MHA), followed by 24 h of incubation at 37°C. table ft1 table-wrap mode="anchored" t5 caption a7 Species Strain a Relevant properties Source or reference Staphylococcus aureus Western Samoan Phage Pattern A (WSPPA) * MRSA ST30 13 ME230 * MSSA, isogenic ΔSCC mec mutant of WSPPA M. Ender b SA113 (ATCC 35556) † MSSA, production of PIA-mediated biofilm under aerobic conditions 14 Staphylococcus epidermidis 1457 ‡ Efficient biofilm producer 17 1457 Δ ica ‡ Isogenic Δ ica mutant, devoid of PIA-mediated biofilm 17 Mycobacterium tuberculosis ATCC 27294 (H37Rv) § Rifampin-sensitive strain 15 ATCC 35838 (H37Rv-RIF-R) ‡ Rifampin-resistant strain Streptococcus pyogenes ATCC 19615 § Laboratory strain isolated from human 16 Streptococcus pneumoniae TIGR4 (JNR.7/87) Encapsulated, virulent strain Enterococcus faecalis ATCC 19433 § Laboratory strain Pseudomonas aeruginosa PA01 ¶ Laboratory strain isolated from human Escherichia coli ATCC 25922 Human clinical isolate Candida albicans SC5314 ∥ Laboratory strain isolated from human Candida glabrata t608919 ‡ Clinical isolate This study Candida krusei ATCC 6258 ‡ Reference laboratory strain isolated from human Open in a separate window a Sources: *, B. Berger-Bächi, Zürich, Switzerland; †, F. Götz, Tübingen, Germany; ‡, D. Mack, Swansea, United Kingdom; §, R. Frei, Basel, Switzerland; ¶, U. Jenal, Basel, Switzerland; ∥, S. Leibundgut-Landmann, Zürich, Switzerland. b M. Ender, ETH Zürich, Switzerland (unpublished data).

Techniques: In Vitro, Bacteria